A pre-tRNA carrying intron features typical of Archaea is spliced in yeast.

نویسندگان

  • Gianfranco Di Segni
  • Lodovica Borghese
  • Silvia Sebastiani
  • Glauco P Tocchini-Valentini
چکیده

Archaeal pre-tRNAs are characterized by the presence of the bulge-helix-bulge (BHB) structure in the intron stem-and-loop region. A chimeric pre-tRNA was constructed bearing an intron of the archaeal type and the mature domain of the Saccharomyces cerevisiae suppressor SUP4 tRNA(Tyr). This pre-tRNA(ArchEuka) is correctly cleaved in several cell-free extracts and by purified splicing endonucleases. It is also cleaved and ligated in S. cerevisiae cells, providing efficient suppression of nonsense mutations in various genes.

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عنوان ژورنال:
  • RNA

دوره 11 1  شماره 

صفحات  -

تاریخ انتشار 2005